Influence of pN2 and pD2 on HD formation by various nitrogenases
- 13 September 1983
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 22 (19) , 4472-4480
- https://doi.org/10.1021/bi00288a019
Abstract
Formation of HD from D2 was demonstrated with nitrogenase preparations from Azotobacter vinelandii, Clostridium pasteurianum, Klebsiella pneumoniae and Azospirillum sp. The formation of HD from D2 is a general property of nitrogenases. The nitrogenases differ in their Ki values for D2 (N2 fixation) and in their rates of catalyzing HD formation; among the nitrogenases tested, C. pasteurianum nitrogenase had the lowest activity for formation of HD. When contaminating N2 was removed from the atmospheres above reaction mixtures, less than 1% of the total electron flux in the system was directed to HD formation; N2-independent HD formation is apparently not significant. A working hypothesis is suggested that operates without invoking an N2-independent reaction for forming HD.This publication has 16 references indexed in Scilit:
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