The sequences of two nuclear genes and a pseudogene for tRNAPro from the higher plant Phaseolus vulgaris
- 1 January 1986
- journal article
- research article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 7 (3) , 207-212
- https://doi.org/10.1007/bf00021332
Abstract
A genomic bank of nuclear DNA (nDNA) from the higher plant Phaseolus vulgaris, constructed using the lambda EMBL-4 vector, has been screened for the presence of tRNA genes. One of the many positive recombinants was found to hybridise several times stronger than the other positives, and has been shown to contain several tRNA genes. We report the structure of two nuclear tRNA genes for tRNAPro, namely tRNAPro(UGG) and tRNAPro(AGG), and that of a ‘pseudogene’ for tRNAPro. This ‘pseudogene’, despite showing 95% homology with the other tRNAPro species presented here, has several features which are likely to affect its transcription or its functioning as a tRNA.Keywords
This publication has 23 references indexed in Scilit:
- Point mutations in the 5′ ICR and anticodon region of aDrosophilatRNAArggene decreasein vitrotranscriptionNucleic Acids Research, 1985
- Compilation of sequences of tRNA genesNucleic Acids Research, 1985
- Genes and pseudogenes in a reiterated rat tRNA gene clusterNucleic Acids Research, 1984
- Chapter 3 Transcription By RNA Polymerase IIIPublished by Elsevier ,1983
- Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elementsNature, 1981
- Nucleotide sequences in Xenopus 5S DNA required for transcription terminationCell, 1981
- Analysis of a Drosophila tRNA gene cluster: Two tRNALeu genes contain intervening sequencesCell, 1981
- Mutations of the yeast SUP4 tRNATyr Locus: Transcription of the mutant genes in vitroCell, 1980
- Analysis of a drosophila tRNA gene clusterCell, 1980
- Studies on plant deoxyribonucleic acidsArchives of Biochemistry and Biophysics, 1968