Vaccinia virus late transcripts generated in vitro have a poly(A) head.
Open Access
- 1 April 1988
- journal article
- research article
- Published by Wiley in The EMBO Journal
- Vol. 7 (4) , 1183-1190
- https://doi.org/10.1002/j.1460-2075.1988.tb02929.x
Abstract
A cell free system mediating accurate transcription of vaccinia virus genes was established using lysates of cells in the late phase of infection. Vaccinia late genes are faithfully transcribed in this extract whereas cellular pol II and pol III promoters are not recognized. The late viral transcripts contain a poly(A) head of approximately 35 nt at the 5′ end which is not co‐linearly encoded in the externally added template. The transcripts obtained in vitro are indistinguishable from the mature in vivo RNAs. The poly(A) head is synthesized de novo and its formation appears to be directly coupled to the transcription of the gene. The synthesis of the poly(A) head transcripts in vitro is consistent with a proposed slippage model.This publication has 35 references indexed in Scilit:
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