Current Status of Retroviral Vector Mediated Gene Transfer into Human Hematopoietic Stem Cells
- 1 January 2001
- journal article
- review article
- Published by Taylor & Francis in Leukemia & Lymphoma
- Vol. 41 (1-2) , 1-18
- https://doi.org/10.3109/10428190109057950
Abstract
Genetic modification of hematopoietic stem cells (HSCs) has been proposed as a treatment strategy for a variety of hematologic diseases, tracking marked cells or conferring resistance to chemotherapeutic agents. Despite early enthusiasm, the results of clinical studies involving gene transfer into HSCs have not resulted in therapeutic benefits for the vast majority of treated patients. This review describes the limitations and advances that have been made in the areas of gene transfer vectors, identification of the appropriate HSCs to target for genetic modifications and the methods used to perform gene transfer.Keywords
This publication has 147 references indexed in Scilit:
- An in vitro system for efficiently evaluating gene therapy approaches to hemoglobinopathiesGene Therapy, 2000
- Human Cord Blood CD34+CD38- Cell Transduction via Lentivirus-Based Gene Transfer VectorsHuman Gene Therapy, 1999
- Green Fluorescent Protein as a Selectable Marker of Fibronectin-Facilitated Retroviral Gene Transfer in Primary Human T LymphocytesHuman Gene Therapy, 1999
- Transduction of hematopoietic stem cells in humans and in nonhuman primatesThe International Journal of Cell Cloning, 1997
- The 30/35 kDa chymotryptic fragment of fibronectin enhances retroviral-mediated gene transfer in purified chronic myelogenous leukemia bone marrow progenitorsLeukemia, 1997
- In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral VectorScience, 1996
- Retroviral Mediated Transfer of the Human Multidrug Resistance Gene (MDR-1) into Hematopoietic Stem Cells During Autologous Transplantation after Intensive Chemotherapy for Metastatic Breast Cancer. National Institutes of Health, Bethesda, MarylandHuman Gene Therapy, 1994
- Helper virus induced T cell lymphoma in nonhuman primates after retroviral mediated gene transfer.The Journal of Experimental Medicine, 1992
- Hematopoietic Reconstitution in a Patient with Fanconi's Anemia by Means of Umbilical-Cord Blood from an HLA-Identical SiblingNew England Journal of Medicine, 1989
- Conditions controlling the proliferation of haemopoietic stem cells in vitroJournal of Cellular Physiology, 1977