THE FRACTIONATION OF EXOGENOUS AND ENDOGENOUS THYROID-STIMULATING HORMONE FROM HUMAN AND RAT PLASMA AND TISSUES*

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Abstract
A method of fractionation is presented which permits the recovery of thyrotropic activity from serum, plasma and tissue homogenates even when present in concentra-tions as low as 0.002 mU per ml. A single application of the acid-salt fractionation method permits discard of about 75 percent of the plasma proteins. After a second fractionation, as much as 95 percent of the plasma proteins may be discarded without significant loss of thyrotropic activity. By this method, the potency of normal rat serum was found to be 0.02 mU per ml, and that of normal human plasma only 0.002 mU per ml. In hypothyroid rats and patients the levels were elevated, and in some patients with Graves'' disease the levels were as high as 6000 times normal.

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