Quantifying dye tracers in soil profiles by image processing

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Abstract
Summary: Developing and testing models for solute transport in the field requires experimental data on the spreading of solutes in the soil. Obtaining such data is costly, and a substantial part of the total costs is in the preparation and chemical analysis of the tracing compounds in the gathered samples. We developed a cheap method to quantify the concentration of the mobile dye tracer Brilliant Blue FCF from digitized photographs of stained soil profiles, and we have tested it in the field. Soil sampling and chemical analyses were necessary only to establish a calibration relation between the dye content and the colour of the soil. The digital images were corrected for geometrical distortions, varying background brightness, and colour tinges, and then they were analysed to determine the soil colour at sampling points in the profiles. The resident concentration of the dye was modelled by polynomial regression with the primary colours red, green, blue and the soil depth as explanatory variables. Concentration maps of Brilliant Blue were then computed from the digitized images with a spatial resolution of 1 mm. Validation of the technique with independent data showed that the method predicted the concentration of the dye well, provided the corrected images contained only the colours included in the calibration.