The Use of Monosaccharides, Disaccharides, and Trisaccharides in Synthetic Diluents for the Storage of Ram Spermatozoa At 37°C and 5°C

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Abstract
Using synthetic semen dilutents based on 20 m[image] phosphate buffer, 31 m[image] NaCl, 0.8% non-dialysable skim milk solids, plus antibiotics, and 185 m[image] of the sugars ribose, arabinose, xylose, glucose, mannose, fructose, galactose, rhamnose, maltose, lactose, sucrose, or raffinose, it wasKfound that ram spermatozoa survive best at 37[degeree]C in diluents containing glucose, mannose, fructose, or sucrose; however, at 5[degeree]C ribose, arabinose, xylose, and galactose were the sugars of choice. Increasing replacement of fructose in the diluent with up to 185 m[image] of these sugars resulted in increased survival at the respective temperatures. Part replacement of 185 m[image] fruetose in the range 7.75-62 m[image] with the sugars most favourable at 5[degree]C was of little benefit. No effect of changes in osmotic pressure was noted using varying concentrations of the sugars to give tonicitles of 0.9, 1.0 and 1.1 relative to 154 m[image] NaCl or 308 m[image] sugar. Increased motility scores and percentages of motile spermatozoa were observed when 17 m[image] fructose was added to ribose and arabinose diluents at 5[degree]C, but not when added to diluents containing xylose, the hexoses, galactose, fructose, and glucose; nor for any sugar-containing diluent at 37[degree]C.

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