Frozen Boar Spermatozoa: Methods of Thawing Pellets

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Abstract
In four experiments, the effects of pre-thaw delay, method of thawing, temperature of thawing solution and pellet volume on acrosome morphology and motility of boar spermatozoa frozen by the pellet method were examined. Holding the frozen pellets for 0, 1.5, 3 or 4.5 min in a Styrofoam container before transferring them into the thawing solution resulted in maximum post-thaw percentages of normal apical ridge (NAR) acrosomes and motile spermatozoa at 3 minutes. Thawing frozen pellets in solutions resulted in higher post-thaw percentages of NAR acrosomes than thawing in a Teflon-coated pan (P<.005); however, the opposite result was obtained for percentage of motile spermatozoa. Thawing frozen pellets in a solution heated to 50 C resulted in higher percentages of NAR acrosomes and motile spermatozoa than thawing in a solution heated to 40 or 60 C. Pellet volumes of .09, .18 or .27 ml had no effect on the percentage of NAR acrosomes after thawing; however, there was a significant positive linear relationship between pellet volume and motility (P<.005).

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