Chemical characterization of p17gag from human immunodeficiency virus as an N‐terminally myristoylated protein
Open Access
- 1 June 1989
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 182 (2) , 323-326
- https://doi.org/10.1111/j.1432-1033.1989.tb14833.x
Abstract
The N-terminal p17gag protein of the human immunodeficiency virus has been shown to incorporate radioactivity following labelling of infected cell lines with [3H]myristic with [3H]myristic acid. We investigated p17gag to determine whether the incorporated radioactivity was the consequence of N-terminal myristoylation. The virus was purified by density gradient centrifugation after labelling chronically infected H9 cells with [3H]myristic acid. The p17gag was isolated by immunoprecipitation and subjected to partial acid hydrolysis. [3H]Myristoylglycine generated by the hydrolysis was derivatized to 4-(p-nitrobenzylidene)-2-tridecanoyloxazol-5-one and identified against a co-eluting, derivatized, unlabelled N-myristoylglycine standard by reverse-phase high-performance liquid chromatography. This study unequivocally demonstrates that p17gag is an N-myristoylated protein.This publication has 25 references indexed in Scilit:
- HIV F/3' orf encodes a phosphorylated GTP-binding protein resembling an oncogene productNature, 1987
- Myristoylation and the post-translational acquisition of hydrophobicity by the membrane immunoglobulin heavy-chain polypeptide in B lymphocytes.Proceedings of the National Academy of Sciences, 1987
- Purification and characterization of yeast myristoyl CoA:protein N-myristoyltransferase.Proceedings of the National Academy of Sciences, 1987
- Acylation of cellular proteins with endogenously synthesized fatty acidsBiochemistry, 1986
- Nucleotide sequence of the AIDS virus, LAVCell, 1985
- Myristylation of gag-onc fusion proteins in mammalian transforming retrovirusesVirology, 1984
- Myristyl amino-terminal acylation of murine retrovirus proteins: An unusual post-translational protein modificationProceedings of the National Academy of Sciences, 1983
- Identification of the NH2‐terminal blocking group of calcineurin B as myristic acidFEBS Letters, 1982
- n-Tetradecanoyl is the NH2-terminal blocking group of the catalytic subunit of cyclic AMP-dependent protein kinase from bovine cardiac muscle.Proceedings of the National Academy of Sciences, 1982
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970