In situ fluorescence hybridization of Y translations: cytogenetic analysis using probes Y190 and Y431

28 June 2008

journal article
research article
Published by Wiley in Clinical Genetics

Vol. 33 (3) , 156-161
https://doi.org/10.1111/j.1399-0004.1988.tb03431.x

Abstract

Two moderately repetitive DNA probes (Y190 and Y431) and a fluorescent in situ hybridization technique, using a biotin, avidin, anti-avidin system, were employed to investigate a group of patients with Y chromosome abnormalities. In normal male subjects, a bright fluorescent spot could be detected in cells in interphase and on the short arm of the Y chromosome in metaphase spreads. Translocations of DNA fragments of the short arm of the Y chromosome to autosomes 10, 13 and 15 were observed in five patients. In a 45,XX male subject the translocation involved one of the X chromosomes. With this in situ hybridization procedure, bright fluorescent spots were also noticed in uncultured amniotic cells and chorionic cellular elements from male fetuses, thus allowing a rapid and reproducible approach to prenatal fetal sexing.

Keywords

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