Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase.
- 1 March 1971
- journal article
- Vol. 7 (3) , 389-94
Abstract
The virions of Newcastle disease virus (NDV) contained an enzyme that catalyzed the incorporation of ribonucleotides into ribonucleic acid (RNA). Optimal conditions for this polymerase activity were identical to the conditions for the vesicular stomatitis virus (VSV) polymerase, and both enzymes were active for longer times at 32 C than at 37 C. However, the specific activity of the NDV polymerase was less than 3% that of the VSV polymerase. Product RNA species from the NDV and VSV polymerase reactions annealed specifically to the homologous virion RNA species. Transcriptive intermediates containing product RNA attached to the respective virion RNA could be identified in both systems.This publication has 22 references indexed in Scilit:
- Ribonucleic acid synthesis of vesicular stomatitis virusVirology, 1970
- Complementary RNAs in Paramyxovirions and Paramyxovirus-infected CellsNature, 1970
- Self-annealing of Subgroup 2 Myxovirus RNAsNature, 1970
- Initiation of polyribosome formation in poliovirus-infected HeLa cellsJournal of Molecular Biology, 1970
- Replication of Sendai virusVirology, 1968
- Vesicular stomatitis virus RNA: Complementarity between infected cell RNA and RNA's from infectious and autointerfering viral fractionsBiochemical and Biophysical Research Communications, 1968
- Comparative sedimentation coefficients of RNA extracted from plaque-forming and defective particles of vesicular stomatitis virusJournal of Molecular Biology, 1966
- Newcastle disease virus RNAJournal of Molecular Biology, 1966
- Newcastle disease virus RNAJournal of Molecular Biology, 1966
- Isolation of the nucleic acid of Newcastle disease virus (NDV).Proceedings of the National Academy of Sciences, 1965