Characterization of the genetic system of the xylose-fermenting yeast Pichia stipitis
- 1 October 1996
- journal article
- research article
- Published by Springer Nature in Current Microbiology
- Vol. 33 (4) , 237-242
- https://doi.org/10.1007/s002849900106
Abstract
High mutant frequencies indicated that the wild-type strains of Pichia stipitis are haploid. Sporulation ability of these clones pointed to a homothallic life cycle. Mating was induced by cultivation under nutritionally poor conditions on malt extract medium. Conjugation was followed immediately by sporulation. However, hybrids could be rescued by transferring the nascent zygotes to complete medium before meiosis had started. Under rich nutritional conditions, hybrids were mitotically stable and did not sporulate. The segregation pattern of auxotrophic markers of diploid zygotes indicated regular meiosis, although asci contained preferentially spore dyads.Keywords
This publication has 23 references indexed in Scilit:
- Peculiarities of the regulation of fermentation and respiration in the crabtree-negative, xylose-fermenting yeastPichia stipitisApplied Biochemistry and Biotechnology, 1996
- Strain selection, taxonomy, and genetics of xylose-fermenting yeastsEnzyme and Microbial Technology, 1994
- Process parameters and environmental factors affecting d-xylose fermentation by yeastsEnzyme and Microbial Technology, 1994
- Biochemistry and physiology of xylose fermentation by yeastsEnzyme and Microbial Technology, 1994
- Theoretical and practical aspects of ploidy estimation in Pichia stipitisMycological Research, 1994
- Apomixis in Saccharomyces cerevisiae and Other Eukaryotic Micro-organismsPublished by Elsevier ,1990
- Auxotrophic mutants of the yeastTrichosporon adeninovoransJournal of Basic Microbiology, 1989
- A simple technique for random spore analysis of yeasts using nystatinJournal of Microbiological Methods, 1985
- Hybridization of yeasts by protoplast fusion: Early events after fusion inPichia guilliermondiiJournal of Basic Microbiology, 1984
- Rhodosporidium BANNO: Inaktivierende Wirksamkeit von ultraviolettem Licht und N-Methyl-N′-nitro-N-nitrosoguanidin in der HefephaseJournal of Basic Microbiology, 1977