Auxotrophic mutants of the yeastTrichosporon adeninovorans

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Abstract
We have isolated and characterized auxotrophic mutants of Trichosporon adeninovorans, strain PAR-4 to get genetic markers that cover the entire nuclear genome of this thermotolerant yeast of technological interest. The nitrosoguanidine mutagenesis yielded mutants at a high frequency. We detected a broad spectrum of auxotrophic phenotypes in the random mutant samples. Obviously, strain PAR-4 is a haploid or hyper-haploid yeast. In correspondence we determined a low DNA content per cell. In contrast to NG1, UV light was an inefficient mutagen. UV survival curves were without the typical shoulder indicating suppression of repair of UV-induced lethal lesions. Thus, the response of PAR-4 to UV was different from those of Saccharo-myces cerevisiae and other yeasts.

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