The Multiplication of an Influenza C Virus in an Established Line of Canine Kidney (MDCK) Cells
- 1 April 1978
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 39 (1) , 179-181
- https://doi.org/10.1099/0022-1317-39-1-179
Abstract
Summary JJ/50 and four other strains of influenza C virus grew in an established line of canine kidney (MDCK) cells. Multicycle virus growth was markedly enhanced by the addition of trypsin to the culture medium and these viruses could be passaged serially in this system. The addition of appropriate concentrations of trypsin to the agar overlay medium enabled plaquing of influenza C/JJ/50 virus. Titration by plaque assay on MDCK cells was more sensitive than that by intra-amniotic inoculation of fertile hens' eggs.This publication has 1 reference indexed in Scilit:
- Permanent canine kidney (MDCK) cells for isolation and plaque assay of influenza B virusesMedical Microbiology and Immunology, 1975