Observations on the Action of Streptomycin in vitro (I).

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Abstract
A standardized procedure for detn. of sensitivity of microorganisms to streptomycin is described. The sensitivity of an organism to streptomycin is influenced by age of culture, conc. of organisms, growth phase of culture, and constituents of medium used. Providing these factors are held constant, the sensitivity of a given strain will remain constant from day to day. The action of streptomycin is bacteriostatic rather than bactericidal. Its action is inhibited by certain growth stimulating substances such as peptone, as well as by certain reducing substances. The sensitivity of 84 strains belonging to 7 spp. is described. Marked variation in sensitivity exists between different strains within a single species and at times between different cells within a given strain. The sensitivity of 9 strains belonging to 8 spp. is essentially the same when tested against crude streptomycin sulfate, against 3 prepns. of the crystalline CaCl2 double salt of streptomycin, and against a prepn. of streptomycin sulfate prepd. from a crystalline salt. The sensitivity of 4 strains of Eberthella typhosa to certain prepns. of impure streptomycin sulfate is greater than to highly purified streptomycin sulfate.
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