OH-Induced Free Radicals in Uridine Studied by a Method Combining ESR, Spin-Trapping, and Liquid Chromatography
- 1 October 1986
- journal article
- research article
- Published by JSTOR in Radiation Research
- Vol. 108 (1) , 1-11
- https://doi.org/10.2307/3576964
Abstract
Free radicals produced by the reactions of OH radicals with uridine were investigated by a method combining ESR, spin-trapping, and liquid chromatography. A N2O-saturated aqueous solution of uridine, containing 2-methyl-2-nitrosopropane as a spin-trap, was X-irradiated and the resulting spin-adducts were separated by gel permeation chromatography and reverse-phase HPLC. ESR and uv-absorbance spectra obtained from the separated spin-adducts show that 5-yl and 6-yl radicals are produced by OH addition to the 5,6 double bond of the base moiety. It is also shown that radicals due to H abstraction from the sugar moiety of the C-4'' and C-5'' positions are produced.This publication has 7 references indexed in Scilit:
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