Amino Acid Requirements for Growth of Avian Lung Cultures.

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Abstract
Primary chick embryo and maturing cockerel lung cultures were found to grow in a test medium containing dialyzed chick embryo extract, dialyzed bovine serum, andavariety of small molecular constituents. The avian cultures required the 13 amino acids usually found to be essential for mammalian cells. They also were markedly stimulated by blycine or L-serine and by small quantities of L-alanine. Folinic acid and higher levels of folic acidpartially replaced the glycine requirement for optimal growth. Pyruvate, oxalacetate, or alpha-ketoglutarate, replaced the requirement for L-alanine. Pyridoxal phosphate partially replaced the requirement for L-alanine.