Chromosomal Aberrations Induced by Hyponitrite and Hydroxylamine Derivatives2

Abstract
Hydroxylamine and certain of its derivatives induced fragmentations, translocations, and rearrangements of the metaphase chromosomes of mouse embryo and Chinese hamster cells. The compounds included: N-methylhy-droxylamine; N-hydroxyurethan, a carcinogen; hydroxyurea, which exerts a leukopenic action in patients with chronic granulocytic leukemia; N-hydroxyaminoacetic acid, derived from the tumor-inhibitor hadacidin; and hyponitrous acid, which can be formed in situ from hydroxylamines. Since these agents induced main-chain cleavage of isolated deoxyribonucleic acid (DNA) in solution, it is believed that the observed morphological aberrations are due to a direct attack on the DNA of the chromosome.

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