Glycoproteins, enzymatic activities, and b proteins in intercellular fluid extracts from hypersensitive Nicotiana species infected with tobacco mosaic virus

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Abstract
Interecellular fluid b proteins from hypersensitive N. tabacum L. c. Xanthi-nc and N. sylverstris Speg. and Comes infected with tobacco mosaic virus were compared by 2-dimensional (2-D) polyacrylamide gel electrophoresis. Except for missing bands b2, b6.alpha., b6.beta. and b7.beta. the overall 2-D electrophoretic pattern of N. sylvestris intercellular fluid proteins was similar to the one observed with Xanthi-nc tobacco. Intercellar proteins were also studied by chromatography on concanavalin A. Glycoproteins corresponding to b6.alpha. and b7.alpha. proteins of N. tabacum and the b''7.alpha. analog of N. sylverstris were identified. These proteins are probably peroxidase isozymes, as peroxidase activities with the same electrophoretic mobility were detected after polyacrylamide gel electrophoresis. No esterase activity was associated with any b protein band in gels. Esterase activities decreased upon virus infection, but accumulation of b proteins and peroxidase activitive increased.

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