Comparative Effects of Selected Azole Compounds on Trophic and Cystic Stages of Acanthamoeba polyphaga

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Abstract
A clinical isolate of Acunthamoeba polyphagu, associated with Acunthamoebu keratitis, was used for in vitro evaluation of antiamebic activity of selected azole compounds. Those antimicrobials tested included clotrimazole, bifonazole, fluconazole, itraconazole, and ketoconazole. The drugs were tested at varying concentrations on axenic trophic amebas and MgC12,‐induced cysts. On the basis of their effects on the two stages in the ameba life‐cycle, clotrimazole and bifonazole were the more promising of the azoles screened but, at concentrations tested, were amebastatic rather than amebicidal. Other strains of A. polyphaga and species of Acunthamoebu were used for comparing variation in response to these and other antimicrobial agents.