Establishment of a macrophagelike cell line derived from U-937, human histiocytic lymphoma, grown serum-free
- 1 October 1990
- journal article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology
- Vol. 26 (10) , 949-954
- https://doi.org/10.1007/bf02624468
Abstract
A human macrophagelike cell line which grows in serum-free medium was established from a histiocytic lymphoma cell line, U-937. U-937 cells failed to differentiate into macrophagelike cells in serum-free medium plus 12-O-tetradecanoyl phorbol 13-acetate (TPA). Fibronectin and albumin in serum were necessary for differentiation of U-937 ceds into macrophagelike cells in enriched RDF medium supplemented with insulin, transferrin, ethanolamine, selenite, egg yolk lipoprotein (eRDF-ITESL medium). The established cell line exhibited several characteristic properties of macrophage such as nitroblue tetrazolium reduction, phagocytic and α-naphthylbutyrate-esterase activities, and tumor necrosis factor and interleukin 1 production. At present the cells have been continuously maintained in eRDF-ITESL medium through over 150 passages.Keywords
This publication has 12 references indexed in Scilit:
- Novel vitamin D3 derivatives, 26-Homo-Δ22-dehydro-1α, 25(S)-dihydroxyvitamin D3 and 26-Homo-Δ22-dehydro-1α, 25(R)-dihydroxyvitamin D3: Preferential activity in c-myc mRNA production and in induction of phenotypic differentiation of HL-60 cellsArchives of Biochemistry and Biophysics, 1990
- Egg yolk lipoprotein, a new supplement for the growth of mammalian cells in serum-free mediumCytotechnology, 1988
- Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro.The Journal of Experimental Medicine, 1987
- Vitamin D metabolites change the phenotype of monoblastic U937 cells.Proceedings of the National Academy of Sciences, 1983
- INDUCTION OF DIFFERENTIATION OF THE HUMAN HISTIOCYTIC LYMPHOMA CELL-LINE U-937 BY RETINOIC ACID AND CYCLIC ADENOSINE 3'-5'-MONOPHOSPHATE-INDUCING AGENTS1982
- PRIMING OF HUMAN MYELOID LEUKEMIC-CELL LINES HL-60 AND U-937 WITH RETINOIC ACID FOR DIFFERENTIATION EFFECTS OF CYCLIC ADENOSINE 3'-5'-MONOPHOSPHATE-INDUCING AGENTS AND A LYMPHOCYTE-T DERIVED DIFFERENTIATION FACTOR1982
- Cell surface characteristics of human histiocytic lymphoma lines-I. Surface glycoprotein patternsLeukemia Research, 1980
- In vitro activation of a human macrophage-like cell lineNature, 1979
- Establishment and characterization of a human histiocytic lymphoma cell line (U‐937)International Journal of Cancer, 1976
- Cytochemical Identification of Monocytes and GranulocytesAmerican Journal of Clinical Pathology, 1971