EFFECTS OF LUCANTHONE ON SEDIMENTATION PROPERTIES OF DNA FROM HELA-CELLS
- 1 January 1977
- journal article
- research article
- Vol. 37 (7) , 2177-2181
Abstract
Exposure of HeLa [human cervical cancer] cells to lucanthone (3 .mu.g/ml) caused dissociation of a fast-sedimenting duplex DNA complex, as judged by lysis and sedimentation in alkaline sucrose gradients. The effect of lucanthone on the DNA complex resembled that of actinomycin D and ionizing radiation. Protein synthesis inhibitors such as cycloheximide or inhibitors of DNA synthesis such as hydroxyurea did not lead to dissociation of the complex. Lucanthone was more active than hycanthone and 5 other closely related thiaxanthenones tested. Lucanthone promoted X-ray induced denaturation of DNA in intact cells, as judged by their nuclear immunoreactivity to antinucleoside antibodies. Lucanthone did not inhibit repair of X-ray induced DNA single strand breaks.This publication has 5 references indexed in Scilit:
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