Frequency distribution of pre-messenger RNA sequences in polyadenylated and non-polyadenylated nuclear RNA from Friend cells
- 1 January 1980
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (7) , 1643-1660
- https://doi.org/10.1093/nar/8.7.1643
Abstract
Hybridisation of cDNA probes for abundant and rare polysomal polyadenylated RNAs with polyadenylated and non-polyadenylated nuclear RNA from Friend cells indicated that the abundant polysomal polyadenylated RNA sequences were present at a higher concentration in the nucleus than rare polysomal sequences, but at a reduced range of concentrations. The ratio of the concentrations of abundant and rare sequences was about 3 in non-polyadenylated nuclear RNA, 9 in polyadenylated nuclear RNA and 13 in polysomal polyadenylated RNA. This suggests that polyadenylation may play a role in the quantitative selection of sequences for transport to the cytoplasm. Polyadenylation cannot be the only signal for transport, since a highly complex population of nucleus-confined polyadenylated molecules exists, each of which is present on average at less than one copy per cell.Keywords
This publication has 28 references indexed in Scilit:
- Complexity and specificity of polysomal poly(A+) RNA in mouse tissuesBiochemistry, 1976
- Message and non-message sequences adjacent to poly(A) in steady state heterogeneous nuclear RNA of HeLa cellsCell, 1976
- Conservation of cytoplasmic poly(A)-containing RNA in mouse and ratNature, 1975
- Mercurated polynucleotides. New probes for hybridization and selective polymer fractionationBiochemistry, 1975
- A kinetic estimation of base sequence complexity of nuclear poly(A)-containing RNA in mouse friend cellsCell, 1975
- The diversity of the messenger RNA population in growing friend cellsCell Differentiation, 1974
- Globin messenger RNA synthesis and processing during haemoglobin induction in Friend cells. II. Evidence for post-transcriptional control in clone 707Cell Differentiation, 1974
- Globin messenger RNA synthesis and processing during haemoglobin induction in friend cells. I. Evidence for transcriptional control in clone M2Cell Differentiation, 1974
- Sequence divergence of mammalian globin messenger RNANature, 1974
- Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-CelluloseProceedings of the National Academy of Sciences, 1972