Peptide and ester synthesis in organic solvents catalyzed by seryl proteases linked to alumina
- 1 February 1986
- journal article
- research article
- Published by Wiley in Proteins-Structure Function and Bioinformatics
- Vol. 1 (2) , 134-138
- https://doi.org/10.1002/prot.340010205
Abstract
Trypsin and α‐chymotrypsin were immobilized to alumina‐phosphocolamine complex, activated by glutaraldehyde. The immobilized enzymes show a great stability toward organic solvents miscible or immiscible with water. In the presence of a low concentration of water, the immobilized enzymes catalyzed transesterification reactions as well as peptide synthesis. The synthesized peptides were stable toward the immobilized enzymes.Keywords
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