Suppression of a nonsense mutation in mammalian cellsin vivoby the aminoglycoside anthiotics G–418 and paromomycin
Open Access
- 11 September 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 13 (17) , 6265-6272
- https://doi.org/10.1093/nar/13.17.6265
Abstract
Aminoglycoside antibiotics in Escherichia coli and yeast can cause ribosomes to read through stop codons during translation. This can result in the phenotypic suppression of nonsense nutations. We show here for the first time that the aminoglycosides G–418 and paronomycin have similar effects in monkey (COS–7) cells in vivo. Suppression of an amber mutation (TAG) by aninoglycosides can restore the activity of a mutant gene transfected into COS–7 cells to almost 20% of wild type levels.Keywords
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