Divalent cation transport in kidney slices

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Abstract
Correlative studies on transports of Ca2+ and Na+ and the properties of Ca2+ transport were carried out in rat kidney cortex slices. Ouabain had no effect on the Ca2+ transport but did inhibit the Na+ transport. 2,4-Dinitrophenol or anaerobic conditions inhibited Ca2+ and Na+ effluxes but enhanced Ca2+ influx. Sulfhydryl inhibitors i.e., ethacrynic acid, mersalyl and p-chloromercuribenzoic acid suppressed Ca2+ efflux and stimulated Ca2+ influx. Then the kidney cortex slices were treated with the inhibitors there was a reduction in the content of cellular ATP. Apparently Ca2+ transport may be partly independent of Na+ transport Ca2+ efflux may require energy-yielding processes.