Functional domains of transcription factor TFIIB.
- 15 June 1993
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 90 (12) , 5633-5637
- https://doi.org/10.1073/pnas.90.12.5633
Abstract
Transcription factor TFIIB is an essential component of the RNA polymerase II initiation complex. TFIIB carries out at least two functions: it interacts directly with the TATA-binding protein (TBP) and helps to recruit RNA polymerase II into the initiation complex. The sequence of TFIIB reveals a potential zinc-binding domain and an imperfect duplication of approximately 70 amino acids. Mutagenesis of cysteine codons within the putative zinc finger results in mutant proteins that bind normally to TBP but are unable to recruit RNA polymerase II-TFIIF into the initiation complex. Changing the two most highly conserved amino acids in the TFIIB repeats reduces the ability of TFIIB to interact with TBP. Therefore, the two functions of TFIIB can be assigned to two separable functional domains of the protein.Keywords
This publication has 36 references indexed in Scilit:
- [19] Rapid and efficient site-specific mutagenesis without phenotypic selectionPublished by Elsevier ,2004
- Delineation of two functional regions of transcription factor TFIIB.Proceedings of the National Academy of Sciences, 1993
- Transcription initiation complexes and upstream activation with RNA polymerase II lacking the C-terminal domain of the largest subunit.Molecular and Cellular Biology, 1990
- Five intermediate complexes in transcription initiation by RNA polymerase IICell, 1989
- Formation of transcription preinitiation complexes with an amanitin-resistant RNA polymerase II.Journal of Biological Chemistry, 1988
- Function of a yeast TATA element-binding protein in a mammalian transcription systemNature, 1988
- Tat Protein from Human Immunodeficiency Virus Forms a Metal-Linked DimerScience, 1988
- [1] Production of single-stranded plasmid DNAPublished by Elsevier ,1987
- A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.Proceedings of the National Academy of Sciences, 1985
- Purification using polyethylenimine precipitation and low molecular weight subunit analyses of calf thymus and wheat germ DNA-dependent RNA polymerase IIBiochemistry, 1977