Potassium permanganate and tetraethylammonium chloride are a safe and effective substitute for osmium tetroxide in solid-phase fluorescent chemical cleavage of mismatch
- 15 August 1997
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (16) , 3377-3378
- https://doi.org/10.1093/nar/25.16.3377
Abstract
Whilst chemical cleavage of mismatch (CCM) detects all point mutations in DNA, its widespread use has been hampered by the complex multistage methodology and the need for toxic chemicals, in particular osmium tetroxide. Here we show that osmium tetroxide can be replaced by potassium permanganate, giving the same spectrum of mutation detection, but with greater sensitivity. The use of potassium permanganate is compatible with solid phase capture and fluorescent detection, giving a safer method of mutation detection. We present here a comparison of CCM with osmium tetroxide and with potassium permanganate, tested on a complete set of single base pair mismatches and a number of small insertion/deletions.Keywords
This publication has 6 references indexed in Scilit:
- Sensitive and fast mutation detection by solid phase chemical cleavageHuman Mutation, 1996
- Ultrarapid Mutation Detection by Multiplex, Solid-Phase Chemical CleavageGenomics, 1995
- Efficient detection of point mutations on color-codedstrands of target DNA.Proceedings of the National Academy of Sciences, 1994
- Detection of single base mismatches of thymine and cytosine residues by potassium permanganate and hydroxylamine in the presence of tetralkylammonium saltsNucleic Acids Research, 1990
- Reactivity of cytosine and thymine in single-base-pair mismatches with hydroxylamine and osmium tetroxide and its application to the study of mutations.Proceedings of the National Academy of Sciences, 1988
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977