Identification of 1,4‐dihydropyridine binding domains within the primary structure of the α1 subunit of the skeletal muscle L‐type calcium channel

Publisher Website
Google Scholar
Abstract
Calcium channel blockers are drugs that bind to the α1 subunit of L-type calcium channels and selectively inhibit ion movements through these channels. Determination of the mechanism of channel blockade requires localization of drug-binding sites within the primary structure of the receptor. In this study the 1,4-dihydropyridine-binding site of the membrane bound receptor has been identified. The covalently labeled receptor was purified and digested with trypsin. The labeled peptide fragments were immunoprecipitated with sequence-directed antibodies. The data indicate the existence of at least three distinct dihydropyridine-binding domains within the primary structure of the α1 subunit