Loop-mediated Isothermal Amplification Reaction Using a Nondenatured Template
Open Access
- 1 September 2001
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 47 (9) , 1742-1743
- https://doi.org/10.1093/clinchem/47.9.1742
Abstract
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplifies DNA with high specificity, efficiency, and rapidity under isothermal conditions ( 1). The LAMP method requires a set of four specially designed primers and a DNA polymerase with strand displacement activity. The amplification products are stem-loop DNA structures with several inverted repeats of the target and cauliflower-like structures with multiple loops, yielding >500 μg/mL. Although LAMP amplifies DNA under isothermal conditions, the template DNA is heat-denatured. To determine whether LAMP can be performed under isothermal conditions at all steps, we attempted to amplify DNA without using a heat-denatured template.Keywords
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