SEQUENCE DEPENDENT DEAMIDATION RATES FOR MODEL PEPTIDES OF CYTOCHROME C
- 12 January 1974
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 6 (1) , 31-35
- https://doi.org/10.1111/j.1399-3011.1974.tb02355.x
Abstract
The rates of deamidation in pH 7.5, 0.15 M, 37.0°C phosphate buffer of pentapeptide models of the sequences found near asparaginyl and glutaminyl residues in cytochrome c were measured. These rates were used to calculate a sequence‐determined half‐life for non‐deamidated cytochrome c. The sequence‐determined deamidation rates of the pentapeptide models were compared with the in vivo and in vitro deamidation rates of cytochrome c and the in vivo turnover rate of cytochrome c. Deamidation of the peptides during synthesis and purification was also measured. The implications of these measurements for deamidation as a biological molecular timer and deamidation during peptide synthesis are discussed.This publication has 18 references indexed in Scilit:
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