Hormonal Control of Plasminogen Activator Secretion in ZR-75-1 Human Breast Cancer Cells in Culture
- 1 May 1984
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 114 (5) , 1702-1710
- https://doi.org/10.1210/endo-114-5-1702
Abstract
Activity of secreted plasminogen activator (PA) by ZR-75-1 human breast cancer cells in culture is altered by the addition of physiologically relevant concentrations of the hormones 17.beta.-estradiol (E2), insulin and dexamethasone. After 48 h, E2 stimulated PA activity 6-fold at concentrations as low as 10-12 M. This stimulation was prevented by the addition of actinomycin D and cycloheximide. The antiestrogen tamoxifen reduced estrogen stimulation of PA, but had slight stimulatory effects on PA secretion by itself. Insulin (5 .times. 10-10 M) induced a 2-fold increase in PA activity. Effects of insulin and E2 were additive, suggesting independent sites of control of PA production. Dexamethasone (10-8 M) decreased PA activity by 20%, but did not inhibit cell growth at the concentration tested. Secreted PA activity is probably differentially regulated by hormones. Effects of PA and growth do not occur in parallel.This publication has 24 references indexed in Scilit:
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