PURIFICATION AND CHARACTERIZATION OF TOXOPLASMA GONDII TACHYZOITE DNA
- 1 August 1986
- journal article
- research article
- Published by Wiley in Immunology & Cell Biology
- Vol. 64 (4) , 351-355
- https://doi.org/10.1038/icb.1986.37
Abstract
Summary: Toxoplasma gondii DNA from RH strain tachyzoites was purified from contaminating murine DNA by isopycnic density gradient ultracentrifugation in CsCI containing the fluorescent dye Hoechst 33258. Quantitative hybridization studies with DNA from uninfected mice and DNA extracted from oocysts of the ME49 strain of T. gondii confirmed that RH strain DNA could be obtained at least 90% pure with a high recovery. Preliminary characterization studies on such purified DNA showed that it had a G + C content of 50% and was not significantly methylated.This publication has 6 references indexed in Scilit:
- Characterization and in vitro translation of Toxoplasma gondii ribonucleic acidMolecular and Biochemical Parasitology, 1986
- A microanalytical procedure for determination of the base composition of DNAEuropean Journal of Biochemistry, 1985
- Nuclear and mitochondrial DNA of the primate malarial parasite Plasmodium knowlesiMolecular and Biochemical Parasitology, 1985
- Antigenic Differences between Endozoites and Cystozoites of Toxoplasma gondiiJournal of Parasitology, 1983
- Ultrastructure of Excystment of Toxoplasma gondii Oocysts*The Journal of Protozoology, 1978
- DNA Base Composition of Toxoplasma gondii grown in vivoNature, 1967