Chemical Identification of a Tumor-Derived Angiogenic Factor
- 15 May 1987
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 236 (4803) , 843-845
- https://doi.org/10.1126/science.2437656
Abstract
Neoplasms produce substances that induce blood vessel formation (angiogenesis). Fractions from ethanol extracts of the Walker 256 carcinoma were isolated by silica column chromatography and C18 reversed-phase high-performance liquid chromatography. Two of the isolated fractions induced neovascularization when tested in the rabbit corneal micropocket assay. One of the fractions was identified as nicotinamide by desorption-electron impact mass spectrometry, nuclear magnetic resonance spectroscopy, and gas chromatography-mass spectrometry. The second active fraction contained nicotinamide as part of a more complex, as yet unidentified, molecular arrangement. Microgram quantities of commercial nicotinamide induced neovascularization in the corneal micropocket assay and in the chick chorioallantoic membrane assay.This publication has 25 references indexed in Scilit:
- Transforming Growth Factor-α: a More Potent Angiogenic Mediator than Epidermal Growth FactorScience, 1986
- Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cellsBiochemistry, 1985
- Tumor-derived angiogenesis factors from rat Walker 256 carcinoma: an experimental investigation and reviewCellular and Molecular Life Sciences, 1985
- Dipyridamole-induced capillary endothelial cell proliferation in the rat heart--a morphometric investigationCardiovascular Research, 1983
- Angiogenesis Induced by "Normal" Human Breast Tissue: A Probable Marker for PrecancerScience, 1982
- NAD-glycohydrolase activity in Xenopus laevis oocytes and early embryosBiochemical and Biophysical Research Communications, 1982
- Proliferative activity of myocardial capillary wall cells in dipyridamole-treated ratsCardiovascular Research, 1978
- Chemical ionization mass spectrometry of salts and thermally labile organics with field desorption emitters as solids probesAnalytical Chemistry, 1977
- Electron impact ionization mass spectrometry using field desorption activated emitters as solid sample probesAnalytical Chemistry, 1977
- Polymers for the sustained release of proteins and other macromoleculesNature, 1976