Two-Color Fluorescent Labeling of Mineralizing Tissues with Tetracycline and 2,4-Bis[N,N'-Di-(Carbomethyl)Aminomethyl] Fluorescein

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Abstract
Two compounds, tetracycline hydro-chloride and 2,4-bis[N,N[image]-di(carbomethyl)-aminomethyl] fluorescein (DCAF), when administered intraperitoneally and 4 days apart to animals [mice] in the dosage of 25 mg/kg body weight will concentrate in mineralizing tissues and fluoresce in contrasting colors. The tissues are fixed in acetone, Soxhlet extracted with diethyl ether, embedded in Bioplastic, sectioned on a microsection saw, polished to 80-100 [mu] thickness, and viewed under a fluorescent microscope equipped with a Schott BG-12 exciter filter and a Schott GG-1 orange yellow barrier filter. Tetracycline fluoresces yellow; DCAF, green.