Rat Cerebellar Cells in Tissue Culture

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Abstract
Immunohistochemical studies showed that Ig from sera of patients with Chagas'' disease bind specifically to endothelial cells in cryostat sections of the rodent brain. In monolayer cultures of 8 day old rat cerebella both surface and internal labeling of the endothelial cells were detected. In contrast immune sera raised against cold-insoluble globulin (which cross-reacts with fibronectin) also labeled the surface of fibroblasts and the endothelial cells. Chagasic Ig appear to be a useful marker for endothelial cells in neural cell culture.