Cryogenic Preservation of Sperm from Striped Bass

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Abstract
Spermatozoa of striped bass M. saxatilis were successfully cryopreserved, retaining fertilizing capacity for up to 2 yr. Spermatozoa in 14 extenders, in combination with 3 concentrations of 4 cryoprotectants at various sperm:medium ratios, were frozen at different rates and tested. Best results were obtained with extender OH-189 (7.30 g NaCl, 0.38 g KCl, 0.23 g CaCl2.cntdot.2H2O, 5.00 g NaHCO3, 0.41 g NaH2PO4.cntdot.H2O, 0.23 g MgSO4.cntdot.7H2O, 5.00 g fructose, 7.50 g lecithin and 5.00 g mannitol in 968.95 g distilled H2O) combined with 5.0% dimethylsulfoxide and mixed in a 1:4 sperm:medium volume ratio. The highest fertilization obtained with cryopreserved semen was 87.7%. No fertilization was obtained when glycerol, ethylene glycol or propylene glycol were the cryoprotectants. Mean freezing rates > 5.degree. C/min were more effective than slower rates.

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