Modified Oxidation/Fermentation Medium for Use in Identification of Bacterial Fish Pathogens

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Abstract
A medium, designated glucose motility deep (GMD), was developed to determine motility, oxidation, fermentation, and gas production of gram-negative, cytochrome oxidase positive, bacterial fish pathogens in one culture tube. The medium is intended to replace the current glucose oxidation–fermentation test which requires two tubes and a petrolatum overlay. Isolates of Aeromonas hydrophila, A. salmonicida, Pseudomonas fluorescens, Vibrio anguillarum, and Pasteurella piscicida were inoculated into GMD. The oxidation–fermentation metabolism, motility, and gas production of each isolate agreed with results obtained using conventional methods. Key words: bacteriology, bacterial medium, fish disease, fish pathogens, Aeromonas, Pseudomonas, oxidation/fermentation, identification

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