Introduction of a human X-6 translocation chromosome into a mouse teratocarcinoma: investigation of control of HLA-A, B, C expression.

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Abstract
An approach to human developmental biology was developed which exploits somatic cell genetics. The production of the HLA-A,B,C antigens was examined. A human-mouse somatic cell hybrid was constructed which contained a human X-6 chromosome translocation carrying the HLA region; this hybrid was used as a donor of the X-6 translocation in the technique of microcell transfer. The X-6 chromosome recipient was the mouse embryonal carcinoma cell line PCC4. The microcell hybrid MCP-6 retained the embryonal carcinoma phenotype as judged by shape and absence of H-2 expression. Nonetheless, the expression of the HLA-A,B,C genes was not extinguished. HLA-A,B,C antigen production at the cell surface was not detected because this hybrid apparently could not make .beta.2-microglobulin.