ESTABLISHMENT OF A CARCINOEMBRYONIC ANTIGEN-PRODUCING CELL-LINE FROM HUMAN PANCREATIC-CARCINOMA

  • 1 January 1980
    • journal article
    • research article
    • Vol. 71  (5) , 596-601
Abstract
A human pancreatic carcinoma cell line of islet cell origin (QGP-1) was established and maintained for > 2 yr. The parent tumor and the cultured cell line produce carcinoembryonic antigen (CEA), and there is no evidence of hormone secretion from the tumor cells. The epithelioid cells, which had migrated from rounded, irregular cell aggregates, grow as a confluent monolayer with piling up of cells in some areas, and have a population doubling time of 3.5 days. The modal chromosome number was 50. Exponentially growing cultures produce 76.3 ng of CEA/106 cells after 7 days. CEA production was confirmed by immunoperoxidase staining.