EPITHELIAL-CELL LINE WITH CHRONIC POLYOMA INFECTION ESTABLISHED FROM A SPONTANEOUS MOUSE PANCREATIC ADENOCARCINOMA
- 1 January 1978
- journal article
- research article
- Vol. 38 (4) , 969-977
Abstract
The establishment of an epithelial cell line from a mouse pancreatic adenocarcinoma is described. The cell line, designated LTPA, was aneuploid and exhibited many transformed growth properties (rapid growth rate, failure to show density-dependent inhibition of growth, ability to grow in defined medium). A type C oncornavirus was isolated from the culture medium, and EM also revealed the presence of intracisternal type A particles. LTPA cells carried a persistent polyoma infection which produced only low levels of cytopathic effects. A mycoplasmal contamination was also carried. When injected s.c. into Swiss nu/nu mice, LTPA cells formed ductular structures which were destroyed by inflammatory reactions within 3 wk.This publication has 8 references indexed in Scilit:
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