Polyoma Virus and L Cell Relationship. I. Some Characteristics of a Cell Line Persistently Infected With Polyoma Virus2

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Abstract
Clone 929, derived from the strain L cell of Earle, was infected with polyoma virus and maintained in continuous subculture for 2 years, supporting active viral proliferation without evidence of cytopathic effect. Furthermore, there was no demonstrable increase in the neoplastic capacity of the carrier cells, as indicated by their failure to produce solid tumors in C3H mice. Likewise, there was no measurable difference in the ability of the carrier line to produce tumors in the cheek pouches of cortisone-treated hamsters when compared to the parental cell line. Maintenance of the carrier state is not dependent entirely on replacement of infected, dying cells by rapid cellular proliferation or on the addition of viral inhibitors or specific antibody that might be in the serum supplement. Evidence is presented to indicate that the establishment of persistent infection is the usual relationship between polyoma virus and the strain L cell.