A 2-MERCAPTOETHANOL TUBE AGGLUTINATION-TEST FOR DIAGNOSIS OF HEMOPHILUS-PLEUROPNEUMONIAE INFECTION IN PIGS
- 1 January 1984
- journal article
- research article
- Vol. 45 (4) , 715-719
Abstract
A 2-mercaptoethanol (2-ME) tube agglutination (TA) test was developed as an alternative to the complement fixation (CF) test for the detection of antibodies to H. pleuropneumoniae, a causative agent of pleuropneumonia in pigs. Using sera from experimentally infected pigs, herds with confirmed H. pleuropneumoniae infection and from disease-free pigs, the specificity and the sensitivity of the 2-ME-TA test were investigated; results were compared with those obtained using the CF test. The TA test without 2-ME gave 100% nonspecific reaction. The 2-ME-TA test was highly specific and more sensitive than was the CF test. Seemingly, the 2-ME-TA test can be used to monitor herds for H. pleuropneumoniae antibodies instead of the CF test because it was easier to perform. Test procedures for a presumptive herd diagnosis of H. pleuropneumoniae have been proposed.This publication has 8 references indexed in Scilit:
- Evaluation of slide agglutination and ring precipitation tests for capsular serotyping of Haemophilus pleuropneumoniaeJournal of Clinical Microbiology, 1982
- EVALUATION OF THE COMPLEMENT-FIXATION TEST FOR THE DIAGNOSIS OF PLEUROPNEUMONIA OF SWINE CAUSED BY HEMOPHILUS-PLEUROPNEUMONIAE1982
- PREVALENCE OF ANTIBODIES TO HEMOPHILUS-PLEUROPNEUMONIAE IN IOWA SWINE1982
- Studies on haemophilus infection in swine I. Application of the latex agglutination test to the diagnosis of Haemophilus pleuropneumoniae (H. Parahaemolyticus) infectionVeterinary Microbiology, 1981
- PORCINE HAEMOPHILUS-PLEUROPNEUMONIA EPIZOOTIC IN SOUTHWESTERN ONTARIO - CLINICAL, MICROBIOLOGICAL, PATHOLOGICAL AND SOME EPIDEMIOLOGICAL FINDINGS1981
- SEROTYPING AND DETECTION OF HEMOPHILUS-PLEUROPNEUMONIAE BY INDIRECT FLUORESCENT-ANTIBODY TECHNIQUE1981
- SEROLOGIC STUDIES ON PORCINE STRAINS OF HAEMOPHILUS-PARAHAEMOLYTICUS (PLEUROPNEUMONIAE) - EXTRACTION OF TYPE-SPECIFIC ANTIGENS1979
- Brucella -Agglutinating Antibodies: Relation of Mercaptoethanol Stability to Complement FixationScience, 1964