Enzymic and chemical structure mapping of mouse 28S ribosomal RNA-contacts in 5.8S ribosomal RNA

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Abstract
Secondary structure mapping experiments using S1 nuclease, RNase T1, and diethyl pyrocarbonate as conformational probes have identified those regions in mouse 5.8S rRNA containing major sites of interaction with 28S rRNA. One site encompasses the 3''-terminal 20 nucleotides and corresponds to the region identified previously as a component of an RNase-resistant 5.8S/28S rRNA junction complex. A second site, located at the 5'' terminus, was not defined precisely but is believed to involve .apprx. 20-30 nucleotides. The existence of these sites of interaction is supported by comparing sequences of eukaryotic 5.8S and 28S rRNA with those of the prokaryotic 23S rRNA. Evidence for the occurrence of at least 3 helical regions in the central portion of the mouse 5.8S rRNA molecule is also presented.