A method for spatio‐temporal (4‐D) data representation in confocal microscopy: application to neuroanatomical plasticity
- 1 January 1993
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 169 (1) , 27-31
- https://doi.org/10.1111/j.1365-2818.1993.tb03275.x
Abstract
This paper describes a new method for data representation and visualization in four dimensions (three dimensions plus time). Sequential volumes, exhibiting morphological activity, are acquired non-invasively with a confocal scanning laser microscope, where each data set corresponds to a time sample. A pipelined processing includes packing of volumes and specific volume rendering techniques. Subsequent processing in HIS (hue, intensity, saturation)l colour space combines functional, packed images with shaded three-dimensional views. As a result, even subtle changes in morphology become visible and computational time is saved. Experimental findings obtained from investigations of synaptic plasticity in cultured retinal tissue are reported.Keywords
This publication has 9 references indexed in Scilit:
- Visualization of cardiovascular nuclear medicine tomographic perfusion studiesPublished by Institute of Electrical and Electronics Engineers (IEEE) ,2002
- Visualization of cardiac magnetic resonance images with color encoded 2D and 3D functional imagesPublished by Institute of Electrical and Electronics Engineers (IEEE) ,2002
- Data representation and visualization in 4-D microscopyPublished by SPIE-Intl Soc Optical Eng ,1992
- Methodological basis of three-dimensional imaging and visualization in biomedical sciences: a reviewPublished by SPIE-Intl Soc Optical Eng ,1990
- Methodical aspects of 3‐D reconstruction of chromatin architecture in mouse trophoblast giant nucleiJournal of Microscopy, 1990
- Correlation of spinule dynamics and plasticity of the horizontal cell spectral response in cyprinid fish retina: quantitative analysisCell and tissue research, 1990
- Direct 2-D display of 3-D objectsIEEE Computer Graphics and Applications, 1984
- Light-dependent change of cone-horizontal cell interactions in carp retinaBrain Research, 1984
- Light-dependent plasticity of the morphology of horizontal cell terminals in cone pedicles of fish retinasJournal of Neurocytology, 1980