Tetrameric structure of the nonactivated glucocorticoid receptor in cell extracts and intact cells
- 5 December 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 241 (1-2) , 234-238
- https://doi.org/10.1016/0014-5793(88)81068-8
Abstract
Mouse lymphoma cells contain a nonactivated glucocorticoid receptor of M r∼330000 which is heteromeric in nature and is unable to bind to DNA. Following affinity labeling of the steroid-binding subunit and subsequent cross-linking with dimethyl suberimidate at various times either in cell extracts or in intact cells, a series of labeled bands was detected in SDS gels. From the molecular masses of completely and partially cross-linked complexes we conclude that the large nonactivated receptor is a tetramer composed of two 90 kDa subunits, one 50 kDa polypeptide and one steroid-binding subunit.Keywords
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