The influence of divalent cations on the stability of human rotavirus
- 1 March 1981
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 67 (1) , 1-9
- https://doi.org/10.1007/bf01314596
Abstract
The influence of divalent cations on the stability of human rotavirus was investigated using the indirect immunofluorescence (FA) technique in LLC-MK2 cells to titrate infectivity. Rotavirus infectivity was stabilized by calcium and strontium ions in solution but not by magnesium ions. Rotavirus isolates were found to be much less stable at 37° C than at +4° C or 20° C. A study of virus morphology at intervals during the course of the experiment and treatment with the chelating agents EDTA and EGTA suggests that loss of infectivity coincides with the removal of the outer capsid layer and that calcium may be required to maintain virus integrity.Keywords
This publication has 10 references indexed in Scilit:
- Activation of rotavirus RNA polymerase by calcium chelationArchiv für die gesamte Virusforschung, 1979
- Rotavirus Stability and InactivationJournal of General Virology, 1979
- Serological relationships between rotaviruses from different species as studied by complement fixation and neutralizationArchiv für die gesamte Virusforschung, 1977
- Separation and Infectivity of Two Particle Types of Human RotavirusJournal of General Virology, 1977
- Ribonucleic Acid Polymerase Activity Associated with Purified Calf RotavirusJournal of General Virology, 1977
- Diagnosis of Rotavirus Infection by Cell CultureJournal of Medical Microbiology, 1977
- Characterization of Two Particle Types of Calf RotavirusJournal of General Virology, 1976
- Review of calcium methodologies.1975
- Diagnostic electron microscopy of faecesJournal of Clinical Pathology, 1974
- RELATION BETWEEN VIRUSES FROM ACUTE GASTROENTERITIS OF CHILDREN AND NEWBORN CALVESThe Lancet, 1974