Collection of Hypophysial Stalk Blood in Rats

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Abstract
A method for collecting all the effluent blood of the hypophysial stalk in rats is described. This blood is believed to be free of contamination with extraneous blood and with cerebrospinal fluid. The average duration of the collection periods in a group of 40 adult male rats was 4.6 hr. The range of the collection periods extended from 2.75 to 6 hr. The use of this method as a preliminary procedure in the determination of secretory rates of the hypothalamlc releasing factors is discussed.