INTRACELLULAR LOCALIZATION OF SULFONATED meso‐TETRAPHENYLPORPHINES IN A HUMAN CARCINOMA CELL LINE*

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Abstract
Abstract—The intracellular localization of meso‐tetraphenylporphines sulfonated to different degrees (TPPSn), in a human cervix carcinoma cell line (NHIK 3025), was studied by fluorescence microscopy and fluorescence spectroscopy. After an 18 h incubation, TPPS4, TPPS2aand TPPS2owere localized in extranuclear granules. Studies of cells stained with both TPPS4and acridine orange, which is known to fluoresce red in lysosomes, indicated that these granules were lysosomes. In addition, a fraction of the cellbound TPPS4, TPPS2aand TPPS2oseems to be associated with the plasma membrane. Fluorescence quenching studies of cells doublestained with acridine orange and TPPS4indicated that TPPS4is also localized in the nucleus and in the extralysosomal cytoplasm. The intracellular location of TPPS, differed from that of the other TPPSns studied: In 6 out of 9 experiments fluorescing extranuclear granules were found. A diffuse fluorescence extending from the perinuclear area was also observed.
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