CELLULAR UPTAKE AND RELATIVE EFFICIENCY IN CELL INACTIVATION BY PHOTO ACTIVATED SULFONATED meso‐TETRAPHENYLPORPHINES

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Abstract
Abstract—The cellular uptake, relative fluorescence quantum yields and photosensitizing efficiencies of meso‐tetraphenylporphines sulfonated to different degrees (TPPSn) have been investigated using the human carcinoma cell line NHIK 3025. The efficiencies of these dyes in photoinactivation of cells were highly dependent on the number of sulfonate groups on the derivatives. These differences in phototoxicity were primarily due to different abilities to be taken up by cells, but were also dependent upon the cellular localization of the dyes. TPPS1and TPPS2awere more efficiently taken up by the cells than TPPS2oand TPPS4. Plasma membrane associated TPPS4was less efficient in cell inactivation per quantum of fluorescence emitted than intracellularly located dye. This was also to some extent the case for TPPS1but not for TPPS2aand TPPS2o. The results presented here indicate that TPPS2aand TPPS1are the most promising of the TPPSns for possible future use in photodynamic therapy.